Presentation Year

2017

Depreciated Participant

Alexis D. AguiarChemistryUndergraduate Student,Joshua M. MasseyChemistryUndergraduate Student,Jessica K. AndersenChemistryUndergraduate Student,Edward SandovalChemistryUndergraduate Student,Madelyne H. GreenChemistryUndergraduate Student,Jenny A. CappuccioChemistryFaculty

College or Department

Short Description of your Research or Creative Project (700 characters or less)

Anabaena Sensory Rhodopsin (ASR), a photoactive membrane protein, undergoes light induced conformational change associated with release of a transducer protein, ASRT. This complex is proposed to control transcription. However, DNA binding to ASR has not been demonstrated. We prepared ASR in soluble nanodiscs. Our data show ASR nanodiscs had a retinal absorbance shift (≈548 - 537 nm). DLS of ASR nanodiscs, separated by SEC, displayed two populations (21.9 ± 6.3 and 31.1 ± 12.5 nm). These populations may represent varying oligomeric states. Preliminary data from crosslinking studies suggest ASR exist in a trimeric state. We conclude ASR is stabilized in the nanodiscs, enabling studies of ASR.

Permission to Publish Work

Yes

Node ID

313

Page Classification