Presentation Year

2019

Depreciated Participant

Miranda RodriguezBiology Undergraduate Student,Amanda PopeUndergraduate Student

College or Department

Short Description of your Research or Creative Project (700 characters or less)

At Humboldt State University we aim to create a robust CRISPR/Cas9 toolkit which enables one to overexpress, repress, inhibit transcription and perform double strand DNA cuts allowing one to undertake research on genes associated with cancer, autophagy and neurological diseases. These tools will enable future students, private sector, and academia to investigate a wide range of biological questions. Currently in the CRISPR/Cas9 system there are inherent problems ranging from indel mutations, poor gene locus targeting, low efficiency, and in vivo applications.We built upon the knowledge in Dr. Steele’s lab and chose to perform Hi-Fi assembly to overcome technological limitations that lowered.

Permission to Publish Work

Yes

Node ID

794

Page Classification