Presentation Year

2016

Depreciated Participant

Azariah CoblentzChemistryUndergraduate Student,Chris DeAlbaBiologyUndergraduate Student,Elizabeth BaileyBiologyUndergraduate Student,Wesley JenkinsBiologyFaculty,Jenny A CappuccioChemistryFaculty

College or Department

Short Description of your Research or Creative Project (700 characters or less)

Heavy metal contamination of soils and waterways due to industrial processes, such as mining, continues to be a problem in the U.S., such as Flint Michigan. Microbial surface layers (S-layers) have shown promise for binding and sequestering heavy metals. Modifications to the S-later protein (RsaA), in C. vibrioides, could increase this efficiency. Our modified strains absorbed more lead per cell mass. Strains HCm 021, 027, 028 absorbed 197.78 ±2.14, 231.32 ±3.10, and 168.69 ±22.65 (ppm Pb+2/g cells) versus 90.55 +/- 1.07 (ppm/g) for the wild-type. Future goals of this project include determining association constants of lead to cells, and construction of a bioremediation filter.

Permission to Publish Work

Yes

Node ID

122

Page Classification