Presentation Year

2021

Depreciated Participant

Maia Stubbs BiologyUndergraduate Student,Selena DuttonBiologyUndergraduate Student,Haley HetrickBiologyUndergraduate Student,Ashley AmadorBiologyUndergraduate Student,Jacob SmithBiologyUndergraduate Student,Dr. Jenny A. CappuccioChemistryFaculty

College or Department

Short Description of your Research or Creative Project (700 characters or less)

Membrane proteins (MP) are crucial for cell pathways, but are difficult to study due to hydrophobicity. Nanodiscs (ND) provide a lipid bilayer mimetic enabling MP studies. To improve efficiency, we compared two ND assembly methods for detergent removal, and evaluated how lipid affects the MP, Anabaena Sensory Rhodopsin. ND were assembled with purified ASR, various lipids, Sodium Cholate and Apolipoprotein AI. Electrophoresis was utilized to evaluate complex formation. ASR was successfully isolated and assembled into ND. We found that the biobead method was faster and had less risk of loss, showing it to be the better method. ND provide a promising approach to therapeutics affecting MP.

Permission to Publish Work

Yes

Node ID

979

Page Classification