Presentation Year

2022

Depreciated Participant

Nicole L WomackCollege of Natural Resources & SciencesUndergraduate Student,Hannah CornwellUndergraduate Student,David LopezUndergraduate Student,Dylan Morris Undergraduate Student

College or Department

Short Description of your Research or Creative Project (700 characters or less)

The G-protein coupled receptor (GPCR), CB2, is part of a widespread neuromodulatory system found in all animals and is suspected to play a role in both CNS and immune system functions, yet it is a relatively understudied. We demonstrated a successful method for the expression and purification of the GPCR CB2 protein from BL21(DE3) pLysS competent cells. This method involves the culturing of an E. coli BL21(DE3) pLysS MBP-CB2-6xHis cell line, induction of CB2 receptor expression via IPTG, and the harvest and isolation of expressed CB2 proteins via Ni-NTA Immobilized Metal Affinity Chromatography. This method was proven to be successful based on SDS-page and spectrophotometric analyses.

Permission to Publish Work

Yes

Presentation File Upload

Untitled%20presentation%20%281%29.pdf (16.64 KB)

Node ID

1116

Page Classification