Presentation Year

2022

Depreciated Participant

Leila AmraniBiochemistry Undergraduate Student,Elsa BalfeBiochemistryUndergraduate Student

College or Department

Short Description of your Research or Creative Project (700 characters or less)

Anabaena sensory rhodopsin (ASR) is a prokaryotic photoactive transmembrane G protein coupled receptor extracted from the cyanobacterium Nostoc. The potential for ASR and its transducer, ASRT, to transcribe/translate prokaryotic genes into products by light signals is an extremely attractive possibility for future membrane research. Nanodiscs are a useful tool for studying membrane proteins because they serve to create a stable amphipathic environment. In the present study, 6x-His ASR has been extracted, isolated, purified, and inserted into a MSP nanodisc with ApoA1 and DMPC. We examine ApoA1 insertion into the nanodisc via SDS-PAGE and chemiluminescent western blot analysis.

Permission to Publish Work

Yes

Presentation File Upload

ASR_%20Western%20Blot%20Poster%20FINAL.pptx (15.24 MB)

Node ID

1066

Page Classification