Presentation Year

2019

Depreciated Participant

William CastilloChemistryUndergraduate Student,Madelyne GreenChemistryUndergraduate Student

College or Department

Short Description of your Research or Creative Project (700 characters or less)

Nanodics which are nanolipoprotein particles (NLPs) can be assembled in order to solubilize and study membrane proteins in a water soluble discoidal particle.. Here, NLPs were assembled using two techniques: dialysis & biobead adsorption. These methods differ in the removal of a detergent called cholate. The Biobead method is much faster, but is relatively untested, whereas the dialysis method is in current use, but is fairly time consuming. By demonstrating the effectiveness of both techniques for nanodisc assembly, solubilization of membrane proteins can be optimized. This will ultimately be helpful in studying ASR, a retinal containing membrane protein for Anabaena (Now Nostoc) PCC 7120.

Permission to Publish Work

Yes

Node ID

781

Page Classification