Presentation Year
2021
Depreciated Participant
Annie JensenChemistryUndergraduate Student,Tessa M. BalkowChemistryUndergraduate Student,Vincent D. CalderonChemistryUndergraduate Student,Aaron R. DarlingtonChemistryUndergraduate Student,Madison E. KishineffChemistryUndergraduate Student,Jayden J. LoseeChemistryUndergraduate Student,David A. MoralesChemistryUndergraduate Student,Dr. Jenny A. CappuccioChemistryFaculty
College or Department
Short Description of your Research or Creative Project (700 characters or less)
Utilizing cellulase enzymes can enhance the production of biofuels. In this study, 14 cellulases identified through metagenomic analysis of cow rumen were expressed in E. Coli, purified using immobilized metal affinity chromatography (IMAC), and then assessed for enzymatic activity versus a control cellulase isolated from Aspergillus Niger. To do this, we evaluated the breakdown of carboxymethylcellulose (CMC) in a plate assay with Congo Red detection. 4 with high activity and 2 with low activity were selected for analyzing the effective pH on the enzymatic activity and expanding the research to kinetic analysis. Our results could inform new cellulase design and enhance biofuel production.
Is your project focused on any of the following?
explores the relationship of an environmental issue with society OR economy
Permission to Publish Work
Yes
Node ID
982
Page Classification