Friday, February 28th

Time Event Title
12:00 pm

Softball vs. CSU Chico

HSU Softball Field
4:00 pm-5:00 pm

Biological Sciences Seminar Series

HSU Campus
Dr. Michael B. Smeaton, of Stanford University, presents "Interstrand cross-link repair in mammals involves TopoisomeraseIIβ".

Interstrand cross-links (ICLs) are a type of DNA damage that covalently tether the opposing strands of the DNA helix. ICLs are formed by some of the most commonly used chemotherapeutics, repair of which can result in resistance. ICLs can also form through endogenous mechanisms and, if left unrepaired, can contribute towards aging processes.

The pathways that repair ICLs are diverse, however, the proteins that initiate the repair process remain unknown. Here, we examine pathways of ICL repair available to terminally differentiated cells and the proteins that initiate the repair process. Model, site-specific ICLs were synthesized that either fit perfectly into B-form DNA or cause a high level of helical distortion.

Using mammalian cell extracts, a biochemical activity was uncovered that ‘unhooks’ the ICL, the critical first step in the repair process. The unhooking reaction was damage-specific, ATP/Mg2+ dependent and responded to the levels of helical distortion.

In yeast and bacteria, the nucleotide excision repair (NER) pathway is responsible for the initial unhooking step in ICL repair. However, we found the unhooking activity to be independent of the NER pathway in mammalian cells.

Purification of the proteins responsible for unhooking was achieved through fractionation of cell extracts. Mass spec analysis of the final, active fraction identified topoisomerase II. Humans have two isoforms of topoII, α and β. The topoII catalytic inhibitor, merbarone, was able to abolish the unhooking activity in extracts. Recombinant topoIIβ, but not α, was able to nucleolytically process a purified ICL substrate.

Finally, using a plasmid based host-cell reactivation assay, we
confirm the involvement of topoIIβ in repair of ICLs in vivo. These findings have significant clinical implications in treating chemoresistant tumors with readily available drugs.
7:00 pm

Sexland 2014 Keynote: Virgie Tovar!

Kate Buchanan Room
As part of this years Sexland Candyland: Don't Yuck My Yum

"Keep Your Laws Off My Muffin Top: Fat Bodies, Sex & the Politics of Yuck and Yum"

Virgie Tovar, MA is a body image expert and coach. She is the editor of Hot & Heavy: Fierce Fat Girls on Life, Love and Fashion (Seal Press, November 2012). She holds a Master's degree in Human Sexuality with a focus on the intersections of body size, race and gender. After teaching "Female Sexuality" at the University of California at Berkeley, where she completed a Bachelor's degree in Political Science in 2005, she went onto host "The Virgie Show" (CBS Radio) in San Francisco. Contact her at virgie@virgietovar.com.
8:00 pm

Piano Studio Recital

Fulkerson Recital Hall

Ongoing Events:

Time Event Title
Jan 25th-Mar 22nd

HSU Alumni Centennial Road Show

Various locations, USA
Jan 31st-Mar 2nd

Nightwatch: Works by Ellen Garvens

First Street Gallery
Jan 31st-Mar 2nd

The Universe Unfolded Works by Ananda Oliveri

First Street Gallery
Feb 12th-Mar 5th

Campus Conservation Nationals Dorm Competition

HSU Campus
Feb 13th-Mar 8th

HSU Art Department Centennial "Ten Years Out: Art by HSU Alumni" Exhibition

Reese Bullen Gallery
Feb 13th-Mar 8th

Cyrus Smith Art Installation

Library
Feb 20th-Mar 20th

“Through the Eyes of an Eagle” Art Exhibit

Goudi'ni Gallery
Feb 27th-Mar 1st

Spinning Into Butter

Gist Hall Theater

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